Abstract |
Human parainfluenza virus type 3 (hPIV-3) is one of the leading causes for lower respiratory tract disease in children, with neither an approved antiviral drug nor vaccine available to date. Understanding the catalytic mechanism of human parainfluenza virus haemagglutinin- neuraminidase ( HN) protein is key to the design of specific inhibitors against this virus. Herein, we used (1) H NMR spectroscopy, X-ray crystallography, and virological assays to study the catalytic mechanism of the HN enzyme activity and have identified the conserved Tyr530 as a key amino acid involved in catalysis. A novel 2,3-difluorosialic acid derivative showed prolonged enzyme inhibition and was found to react and form a covalent bond with Tyr530. Furthermore, the novel derivative exhibited enhanced potency in virus blockade assays relative to its Neu2en analogue. These outcomes open the door for a new generation of potent inhibitors against hPIV-3 HN.
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Authors | Larissa Dirr, Ibrahim M El-Deeb, Patrice Guillon, Cindy J Carroux, Leonard M G Chavas, Mark von Itzstein |
Journal | Angewandte Chemie (International ed. in English)
(Angew Chem Int Ed Engl)
Vol. 54
Issue 10
Pg. 2936-40
(Mar 02 2015)
ISSN: 1521-3773 [Electronic] Germany |
PMID | 25676091
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Hemagglutinin Glycoproteins, Influenza Virus
- Neuraminidase
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Topics |
- Catalysis
- Crystallography, X-Ray
- Hemagglutinin Glycoproteins, Influenza Virus
(chemistry, metabolism)
- Magnetic Resonance Spectroscopy
- Neuraminidase
(chemistry, metabolism)
- Parainfluenza Virus 3, Human
(enzymology)
- Proton Magnetic Resonance Spectroscopy
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