G-protein coupled receptor 34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro.

Abstract

BACKGROUND:

Overexpression of G-protein coupled receptor 34 (GPR34) affects the progression and prognosis of human gastric adenocarcinoma, however, the role of GPR34 in gastric cancer development and progression has not been well-determined. The current study aimed to investigate the effect of GPR34 knockdown on the proliferation, migration, and apoptosis of HGC-27 gastric cancer cells and the underlying mechanisms.

METHODS:

The expression of GPR34 in gastric cancer cell line HGC-27 was detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HGC-27 cells were employed to construct the stable GPR34 knockdown cell model in this study. Real-time RT-PCR and Western blotting were applied to validate the effect of short hairpin RNA (ShRNA) on the expression of GPR34 in HGC-27 gastric cells. The proliferation, migration of these cells were examined by Cell Counting Kit-8 and transwell. We also measured expression profile of PI3K/PDK1/AKT and ERK using Western blotting.

RESULTS:

The ShRNA directed against GPR34 effectively inhibited both endogenous mRNA and protein expression levels of GPR34, and significantly down-regulated the expression of PIK3CB (P < 0.01), PIK3CD (P < 0.01), PDK1 (P < 0.01), phosphorylation of PDK1 (P < 0.01), Akt (P < 0.01), and ERK (P < 0.01). Furthermore, GPR34 knockdown resulted in an obvious reduction in HGC-27 cancer cell proliferation and migration activity (P < 0.01).

CONCLUSIONS:

GPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer.

• Authors: Zhong-Tian Jin, Kun Li, Mei Li, Zhi-Gang Ren, Fu-Shun Wang, Ji-Ye Zhu, Xi-Sheng Leng, Wei-Dong Yu
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 128 Issue 4 Pg. 545-9 (Feb 20 2015) ISSN: 2542-5641 [Electronic] China
• PMID: 25673461 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• G-protein-coupled receptor 34
• RNA, Small Interfering
• Receptors, Lysophospholipid

Topics

• Apoptosis (genetics, physiology)
• Blotting, Western
• Cell Line, Tumor
• Cell Proliferation (genetics, physiology)
• Humans
• RNA, Small Interfering (genetics)
• Real-Time Polymerase Chain Reaction
• Receptors, Lysophospholipid (genetics, metabolism)
• Stomach Neoplasms (genetics, metabolism)