[Apocynin relieves inflammation in dextran sulfate sodium-induced ulcerative colitis mice: the role of NOXs-ROS-p38MAPK pathway].

Abstract

The study is aimed to explore the molecular mechanism of the treatment of apocynin in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice. 5% DSS was used to mimic the UC model, and 2% apocynin was applied to treat the UC mice. HE staining was used for histopathological evaluation. Chemiluminescence technique was used to measure reactive oxygen species (ROS) production, and the rate of consumption of NADPH inhibited by DPI was detected to determine the NADPH oxidases (NOXs) activity. Western blot was applied to identify the level of p38MAPK phosphorylation, Griess reaction assay to analyze NO production, immunoenzymatic method to determine prostaglandin E2 (PGE2) production, real time RT-PCR and Western blot to identify the expression of iNOS and COX2, and enzyme linked immunosorbent assay to detect inflammatory cytokines TNF-α, IL-6, IFN-γ, IL-1β. Rat neutrophils were separated, and then ROS production, NOXs activity, NO and PGE2 production, NOX1 and p-p38MAPK expression were detected. Compared with the UC group, apocynin decreased ROS over-production and NOXs activity (P < 0.01), reduced p38MAPK phosphorylation, inhibited NO, PGE2 and cytokines production (P < 0.01). Apocynin also decreased NOXs activity and ROS over-production (P < 0.01), inhibited p38MAPK phosphorylation and NOX1 expression, and reduced NO and PGE2 production (P < 0.01) in separated neutrophils from UC mice. Therefore, apocynin could relieve inflammation in DSS-induced UC mice through inhibiting NOXs-ROS-p38MAPK signal pathway, and neutrophils play an important role.

• Authors: Dan-Dan Wei, Xu-Hong Lin, Hui-Chao Wang, Bin Wang, Chun-Yang Bai, Ya-Qiang Wang, Guo-En Li, Xue-Qun Ren
• Journal: Sheng li xue bao : [Acta physiologica Sinica] (Sheng Li Xue Bao) Vol. 67 Issue 1 Pg. 74-82 (Feb 25 2015) ISSN: 0371-0874 [Print] China
• PMID: 25672629 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetophenones
• Cytokines
• Reactive Oxygen Species
• Dextran Sulfate
• acetovanillone
• NADH, NADPH Oxidoreductases
• p38 Mitogen-Activated Protein Kinases

Topics

• Acetophenones (pharmacology)
• Animals
• Colitis, Ulcerative (chemically induced, drug therapy)
• Cytokines (metabolism)
• Dextran Sulfate
• Inflammation (drug therapy)
• MAP Kinase Signaling System
• Mice
• NADH, NADPH Oxidoreductases (metabolism)
• Neutrophils (metabolism)
• Rats
• Reactive Oxygen Species (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)