Abstract | BACKGROUND: RESULTS: In normoxic conditions, cultured cells produced more than 90% of acetyl-CoA from glucose and glutamine-derived carbon. In hypoxic cells, this contribution dropped, ranging across cell lines from 50% to 80%. Thus, under hypoxia, one or more additional substrates significantly contribute to acetyl-CoA production. (13)C-tracer experiments revealed that neither amino acids nor fatty acids are the primary source of this acetyl-CoA. Instead, the main additional source is acetate. A large contribution from acetate occurs despite it being present in the medium at a low concentration (50-500 μM). CONCLUSIONS:
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Authors | Jurre J Kamphorst, Michelle K Chung, Jing Fan, Joshua D Rabinowitz |
Journal | Cancer & metabolism
(Cancer Metab)
Vol. 2
Pg. 23
( 2014)
ISSN: 2049-3002 [Print] England |
PMID | 25671109
(Publication Type: Journal Article)
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