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Differential expression of miR-31 between inflammatory bowel disease and microscopic colitis.

AbstractBACKGROUND:
Idiopathic inflammatory bowel disease (IBD) and microscopic colitis (MC) are distinct entities. However, patients with intermittent episodes of IBD and MC that are encountered in a clinical setting puzzle clinicians and pathologists. This study examined whether microRNA assisted in the classification of IBD and MC.
DESIGN:
Small RNA was extracted from formalin-fixed, paraffin-embedded (FFPE) colon tissue and qRT-PCR was performed from cohorts of normal control (n=38), ulcerative colitis (n=36), Crohns disease (n=26), collagenous colitis (n=36), lymphocytic colitis (n=30), and patients with intermittent features of IBD and MC (n=6).
RESULTS:
Differential expression of miR-31 distinguished IBD (ulcerative colitis and Crohns disease) from MC (collagenous colitis and lymphocytic colitis), confirming the specificity of miR-31 expression in IBD (P=0.00001). In addition, expression of miR-31 was increased in collagenous colitis compared to that of lymphocytic colitis (P=0.010). Among 6 patients with alternating episodes of IBD and MC, one patient had matching miR-31 expression in different phases (lymphocytic colitis to ulcerative colitis, and then back to collagenous colitis). The other 5 patients had MC-like expression patterns in both MC and IBD episodes.
CONCLUSION:
In summary, IBD and MC have distinct miR-31 expression pattern. Therefore, miR-31 might be used as a biomarker to distinguish between IBD and MC in FFPE colonic tissue. In addition, miR-31 is differentially expressed in colonic tissue between lymphocytic colitis and collagenous colitis, suggesting them of separate disease processes. Finally, patients with alternating IBD and MC episodes represent a diverse group. Among them, the majority demonstrates MC-like miR-31 expression pattern in MC phases, which seems unlikely to support the speculation of MC as an inactive form of IBD. Although the mechanisms deserve further investigation, microRNA is a potentially useful biomarker to differentiate IBD and MC.
AuthorsChen Zhang, Zijin Zhao, Hany Osman, Rao Watson, Ilke Nalbantoglu, Jingmei Lin
JournalMicroRNA (Shariqah, United Arab Emirates) (Microrna) Vol. 3 Issue 3 Pg. 155-9 ( 2014) ISSN: 2211-5374 [Electronic] United Arab Emirates
PMID25665881 (Publication Type: Journal Article)
Chemical References
  • Biomarkers
  • MIRN31 microRNA, human
  • MicroRNAs
Topics
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers (metabolism)
  • Colitis, Microscopic (diagnosis, genetics, metabolism)
  • Colitis, Ulcerative (diagnosis, genetics, metabolism)
  • Crohn Disease (diagnosis, genetics, metabolism)
  • Female
  • Humans
  • Male
  • MicroRNAs (genetics, metabolism)
  • Middle Aged

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