The role of FoxJ2 in the migration of human glioma cells.

Abstract

Previous studies have demonstrated that FoxJ2 (forkhead box J2) is a member of Forkhead Box transcription factors and acts as an important prognostic indicator in human breast cancer. Our study aimed to assess the expression and function in human glioma. Western blot analysis and immunohistochemistry were performed in human glioma tissues. Low FoxJ2 expression was observed in 80 samples and its level was correlated with the grade of malignancy. A strongly positive correlation was observed between FoxJ2 and E-cadherin. Overexpression of FoxJ2 increased E-cadherin expression and decreased vimentin expression. The wound healing and transwell assays showed that overexpression of FoxJ2 significantly inhibited their migration in U87 cells. Consistent with this, knockdown of FoxJ2 promoted cellular motility. In a word, FoxJ2 suppressed cell migration and invasion in glioma, which might be a potential novel molecular targeted therapy for surgery and immune treatment.

• Authors: Xiaojun Qiu, Bin Ji, Lixiang Yang, Qingfeng Huang, Wei Shi, Zongmei Ding, Xiaojuan He, Na Ban, Shaochen Fan, Jianguo Zhang, Ye Tian
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 211 Issue 5 Pg. 389-97 (May 2015) ISSN: 1618-0631 [Electronic] Germany
• PMID: 25661068 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier GmbH. All rights reserved.

Chemical References

• FOXJ2 protein, human
• Forkhead Transcription Factors
• RNA, Small Interfering

Topics

• Adult
• Blotting, Western
• Brain Neoplasms (pathology)
• Cell Movement (physiology)
• Cells, Cultured
• Female
• Fluorescent Antibody Technique
• Forkhead Transcription Factors (metabolism)
• Glioma (pathology)
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Neoplasm Invasiveness (pathology)
• RNA, Small Interfering
• Transfection