[Experimental study of adipose-derived mesenchymal stem cells in the treatment of Crohn's disease].

Abstract

OBJECTIVE:

To explore the efficacy of adipose-derived mesenchymal stem cells (ADMSCs) in a murine model of inflammatory bowel disease, and its potential mechanism.

METHODS:

Murine colitis mouse model of Crohn's disease(CD) was created by trinitrobenzene sulfonic acid(TNBS)-induced colitis. Seventy-five 6-8 weeks female BALB/c mice were randomly divided into 3 groups: control group, TNBS group and ADMSC group. To verify the therapeutic effect of ADMSC, real-time PCR and immunohistochemical staining were performed to measure inflammatory cytokines levels in colon tissues. The 10-day survival statuses were recorded after the infusion of ADMSCs.

RESULTS:

Intraperitoneal injection of ADMSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and increased survival(60% vs. 30%, P<0.05) in the TNBS-induced mouse model of CD. Compared with TNBS group, proinflammatory cytokines, including TNF-α, IL-12 and VEGF of ADMSC group were significantly reduced, with significant increase of IL-10 expression.

CONCLUSION:

ADMSCs can effectively repair the injury of colonitis through down-regulation of proinflammatory cytokines TNF-α, IL-12 and VEGF expression, and up-regulation of anti-inflammatory cytokine IL-10 expression, which may be a potential new alternative of cell-based therapy for CD.

• Authors: Minghao Xie, Xiaosheng He, Jinling Zhu, Zhen He, Xiaowen He, Ping Lan, Lei Lian
• Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery (Zhonghua Wei Chang Wai Ke Za Zhi) Vol. 18 Issue 1 Pg. 58-64 (Jan 2015) ISSN: 1671-0274 [Print] China
• PMID: 25656034 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Trinitrobenzenesulfonic Acid

Topics

• Adipocytes
• Animals
• Colitis
• Crohn Disease
• Cytokines
• Disease Models, Animal
• Down-Regulation
• Female
• Inflammatory Bowel Diseases
• Mesenchymal Stem Cells
• Mice
• Mice, Inbred BALB C
• Trinitrobenzenesulfonic Acid
• Up-Regulation