Abstract |
The adenovirus E4orf4 protein expressed at high levels kills cancer cells but not normal human primary cells. Previous studies suggested that disruption of processes that regulate mitosis may underlie E4orf4 toxicity. Here we have used live imaging to show that E4orf4 induces a slowed defective transit through mitosis, exhibiting a delay or often failure in cytokinesis that may account for an accumulation of G1 tetraploids in the population of dying E4orf4-expressing cells.
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Authors | Neera Sriskandarajah, Paola Blanchette, Thomas J Kucharski, Jose G Teodoro, Philip E Branton |
Journal | Journal of virology
(J Virol)
Vol. 89
Issue 8
Pg. 4685-9
(Apr 2015)
ISSN: 1098-5514 [Electronic] United States |
PMID | 25653433
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015, American Society for Microbiology. All Rights Reserved. |
Chemical References |
- E4orf4 protein, adenovirus
- Viral Proteins
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Topics |
- Cell Line, Tumor
- Flow Cytometry
- Humans
- Mitosis
(physiology)
- Molecular Imaging
(methods)
- Time-Lapse Imaging
- Viral Proteins
(physiology, ultrastructure)
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