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Analysis by live imaging of effects of the adenovirus E4orf4 protein on passage through mitosis of H1299 tumor cells.

Abstract
The adenovirus E4orf4 protein expressed at high levels kills cancer cells but not normal human primary cells. Previous studies suggested that disruption of processes that regulate mitosis may underlie E4orf4 toxicity. Here we have used live imaging to show that E4orf4 induces a slowed defective transit through mitosis, exhibiting a delay or often failure in cytokinesis that may account for an accumulation of G1 tetraploids in the population of dying E4orf4-expressing cells.
AuthorsNeera Sriskandarajah, Paola Blanchette, Thomas J Kucharski, Jose G Teodoro, Philip E Branton
JournalJournal of virology (J Virol) Vol. 89 Issue 8 Pg. 4685-9 (Apr 2015) ISSN: 1098-5514 [Electronic] United States
PMID25653433 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015, American Society for Microbiology. All Rights Reserved.
Chemical References
  • E4orf4 protein, adenovirus
  • Viral Proteins
Topics
  • Cell Line, Tumor
  • Flow Cytometry
  • Humans
  • Mitosis (physiology)
  • Molecular Imaging (methods)
  • Time-Lapse Imaging
  • Viral Proteins (physiology, ultrastructure)

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