Abstract | BACKGROUND:
Follicle-stimulating hormone receptor (FSHR) is expressed on the endothelial surface of blood vessels associated with solid tumor periphery, where angiogenesis is known to occur. The correlation between FSHR expression and formation of new peritumoral vessels has not been previously investigated. METHODS: We used immunohistochemical techniques involving specific antibodies to detect FSHR and the endothelial markers (CD34, VEGFR2, and D2-40) in tissue samples from 83 patients with lymph node-negative, invasive breast cancer representing four main clinical treatment groups: HR+/HER2-, HR+/HER2+, HR-/HER2+ and triple-negative. RESULTS: The FSHR+ vessels were exclusively located at breast cancer periphery, in a layer that extended 2 mm into and 5 mm outside of the tumor. The percentage of blood vessels expressing FSHR reached a maximum of 100% at the demarcation line between the tumor and the normal tissue. Common among FSHR+ vessels, regardless of breast cancer type, were the high densities of arterioles and venules (6.4 ± 1.4 and 13.9 ± 2.1 vessels/mm(2), respectively). These values were 3-fold higher that those noticed for CD34+ arterioles and venules associated with normal breast tissue located at a distance greater than 10 mm outside the tumors. The average density of FSHR+ and CD34+ blood vessels as well as of D2-40+ lymphatic vessels did not differ significantly among breast cancer subgroups. FSHR+ vessels did not express VEGFR2. The endothelial FSHR expression correlated significantly with the peritumoral CD34+ vessels' density (p < 0.001) and tumor size (p = 0.01). CONCLUSION:
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Authors | François Planeix, Mohammad-Ahsan Siraj, François-Clément Bidard, Blaise Robin, Christophe Pichon, Xavier Sastre-Garau, Martine Antoine, Nicolae Ghinea |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 34
Pg. 12
(Feb 05 2015)
ISSN: 1756-9966 [Electronic] England |
PMID | 25652007
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Receptors, FSH
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Topics |
- Biomarkers
- Breast Neoplasms
(metabolism, pathology)
- Endothelial Cells
(metabolism)
- Female
- Gene Expression
- Humans
- Immunohistochemistry
- Neoplasm Grading
- Neovascularization, Pathologic
(genetics, metabolism)
- Receptors, FSH
(genetics, metabolism)
- Vascular Remodeling
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