Abstract |
Sorghum responds to the ingress of the fungal pathogen Colletotrichum sublineolum through the biosynthesis of 3-deoxyanthocyanidin phytoalexins at the site of primary infection. Biosynthesis of 3-deoxyanthocyanidins in sorghum requires a MYB transcription factor encoded by yellow seed1 (y1), an orthologue of the maize gene pericarp color1 (p1). Maize lines with a functional p1 and flavonoid structural genes do not produce foliar 3-deoxyanthocyanidins in response to fungal ingress. To perform a comparative metabolic analysis of sorghum and maize 3-deoxyanthocyanidin biosynthetic pathways, we developed transgenic maize lines expressing the sorghum y1 gene. In maize, the y1 transgene phenocopied p1-regulated pigment accumulation in the pericarp and cob glumes. LC-MS profiling of fungus-challenged Y1-maize leaves showed induction of 3-deoxyanthocyanidins, specifically luteolinidin. Y1-maize plants also induced constitutive and higher levels of flavonoids in leaves. In response to Colletotrichum graminicola, Y1-maize showed a resistance response.
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Authors | Farag Ibraheem, Iffa Gaffoor, Qixian Tan, Chi-Ren Shyu, Surinder Chopra |
Journal | Molecules (Basel, Switzerland)
(Molecules)
Vol. 20
Issue 2
Pg. 2388-404
(Jan 30 2015)
ISSN: 1420-3049 [Electronic] Switzerland |
PMID | 25647576
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Anthocyanins
- Flavonoids
- Plant Proteins
- Transcription Factors
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Topics |
- Anthocyanins
(metabolism)
- Colletotrichum
(physiology)
- Disease Resistance
- Flavonoids
(metabolism)
- Host-Pathogen Interactions
- Pigmentation
- Plant Diseases
(microbiology)
- Plant Leaves
(genetics, metabolism, microbiology)
- Plant Proteins
(biosynthesis, genetics)
- Plants, Genetically Modified
- Sorghum
(genetics)
- Transcription Factors
(biosynthesis, genetics)
- Zea mays
(genetics, metabolism, microbiology)
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