An adjuvanted, tetravalent dengue virus purified inactivated vaccine candidate induces long-lasting and protective antibody responses against dengue challenge in rhesus macaques.

Abstract

The immunogenicity and protective efficacy of a candidate tetravalent dengue virus purified inactivated vaccine (TDENV PIV) formulated with alum or an Adjuvant System (AS01, AS03 tested at three different dose levels, or AS04) was evaluated in a 0, 1-month vaccination schedule in rhesus macaques. One month after dose 2, all adjuvanted formulations elicited robust and persisting neutralizing antibody titers against all four dengue virus serotypes. Most of the formulations tested prevented viremia after challenge, with the dengue serotype 1 and 2 virus strains administered at 40 and 32 weeks post-dose 2, respectively. This study shows that inactivated dengue vaccines, when formulated with alum or an Adjuvant System, are candidates for further development.

• Authors: Stefan Fernandez, Stephen J Thomas, Rafael De La Barrera, Rawiwan Im-Erbsin, Richard G Jarman, Benoît Baras, Jean-François Toussaint, Sally Mossman, Bruce L Innis, Alexander Schmidt, Marie-Pierre Malice, Pascale Festraets, Lucile Warter, J Robert Putnak, Kenneth H Eckels
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 92 Issue 4 Pg. 698-708 (Apr 2015) ISSN: 1476-1645 [Electronic] United States
• PMID: 25646261 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © The American Society of Tropical Medicine and Hygiene.

Chemical References

• Adjuvants, Immunologic
• Antibodies, Neutralizing
• Antibodies, Viral
• Dengue Vaccines
• RNA, Viral
• Vaccines, Inactivated

Topics

• Adjuvants, Immunologic
• Animals
• Antibodies, Neutralizing (biosynthesis)
• Antibodies, Viral (biosynthesis)
• Chlorocebus aethiops
• Dengue (prevention & control, virology)
• Dengue Vaccines (administration & dosage, immunology)
• Dengue Virus (immunology)
• Disease Models, Animal
• Female
• Macaca mulatta
• Male
• RNA, Viral (blood)
• Random Allocation
• Time Factors
• Vaccination
• Vaccines, Inactivated (administration & dosage, immunology)
• Vero Cells
• Viremia (prevention & control, virology)