Which type of conditioning method protects the spinal cord from the ischemia-reperfusion injury in 24 hours?

Abstract

OBJECTIVE:

This study was designed to test the effects of different types of preconditioning and postconditioning methods on spinal cord protection following aortic clamping.

METHODS:

The animals (rabbits) were divided into sham-operated, ischemic preconditioning, remote ischemic preconditioning, simultaneous aortic and ischemic remote preconditioning, and ischemic postconditioning groups. After neurological evaluations, ultrastructural analysis and immunohistochemical staining for caspase-3 were evaluated after 24 h following ischemia.

RESULTS:

The neurological outcomes of the remote ischemic preconditioning (4.2 ± 0.4) and ischemic postconditioning (4.6 ± 0.8) groups were significantly improved when compared with the ischemia group (2.2 ± 04). The immunohistochemical analysis revealed that the lowest percentage of apoptosis was in-group ischemic preconditioning at 12.5 ± 30.6%. In the comparison of intracellular edema in an ultrastructural analysis, the ischemic preconditioning and ischemic postconditioning groups had significantly lower values than the ischemia group.

CONCLUSION:

The conditioning methods attenuate ischemia-reperfusion injury for spinal cord injury. Ischemic and remote preconditioning and also postconditioning methods are simple to perform and inexpensive.

• Authors: Ali Sapmaz, A Tulga Ulus, Nilüfer N Turan, F Figen Kaymaz, Hija Yazıcıoğlu, Siyar Ersöz, Erdal Simsek, Cüneyt Köksoy
• Journal: Vascular (Vascular) Vol. 23 Issue 6 Pg. 614-21 (Dec 2015) ISSN: 1708-539X [Electronic] England
• PMID: 25646020 (Publication Type: Comparative Study, Journal Article)
• Copyright: © The Author(s) 2015.

Chemical References

• Caspase 3

Topics

• Animals
• Aorta, Abdominal (physiopathology, surgery)
• Apoptosis
• Axillary Artery (physiopathology, surgery)
• Caspase 3 (metabolism)
• Constriction
• Disease Models, Animal
• Ischemic Postconditioning (methods)
• Ischemic Preconditioning (methods)
• Motor Activity
• Rabbits
• Regional Blood Flow
• Reperfusion Injury (metabolism, pathology, physiopathology, prevention & control)
• Spinal Cord (blood supply, metabolism, ultrastructure)
• Spinal Cord Ischemia (metabolism, pathology, physiopathology, prevention & control)
• Time Factors