Abstract |
Solasonine, a known glycoalkaloid, is a potential anti- cancer agent. In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of solasonine in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an ACQUITY HSS T3 (100×2.1mm, 1.8μm) column with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1-500ng/mL (R(2)>0.994) with a low limit of detection (0.1ng/mL) and lower limit of quantification (0.5ng/mL). The extraction recovery was in the range of 92.4-94.9% for solasonine and 91.9% for dendrobine (internal standard, IS). The intra- and inter-day precision was below 9.8% and accuracy was from 86.0% to 94.3%. No notable matrix effect and astaticism was observed for solasonine. The method has been successfully applied to a pharmacokinetic and bioavailability study of solasonine in rats for the first time, which provides the basis for the further development and application of solasonine.
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Authors | Yuanyuan Chen, Shuangshuang Zhang, Dahui Chen, Mi Zhou, Jing Zheng, Zheng Xiang |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 985
Pg. 1-5
(Mar 15 2015)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 25645955
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Chemical References |
- Solanaceous Alkaloids
- alpha-solamargine
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Topics |
- Animals
- Biological Availability
- Chromatography, High Pressure Liquid
(methods)
- Limit of Detection
- Linear Models
- Male
- Rats
- Rats, Sprague-Dawley
- Reproducibility of Results
- Solanaceous Alkaloids
(blood, chemistry, pharmacokinetics)
- Tandem Mass Spectrometry
(methods)
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