The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella typhimurium infection.

Abstract

Resistance is increasing to several critical antimicrobials used to treat Salmonella typhimurium infection, urging people to search for new antimicrobial agents. In this work, we reported the possibility of a potent antimicrobial peptide cathelicidin-BF found in the venom of the snake Bungarus fasciatus in treating Salmonella typhimurium infection. We tested its activity in biological fluids and in vivo using a mouse model of Salmonella typhimurium infection, and examined the effect of cathelicidin-BF on Salmonella invasion to epithelial cells. In addition, the biodistribution of cathelicidin-BF was evaluated by using in vivo optical imaging. The results revealed that cathelicidin-BF was unstable in gastrointestinal tract, but retained substantially active in murine serum. Cathelicidin-BF attenuated the clinical symptoms of Salmonella infected-mice, significantly reduced the number of internalized Salmonella and attenuated Salmonella-induced decreases in TER in epithelial cells. Our results provide a first indication for the potential of cathelicidin-BF as a novel therapeutic option for salmonellosis.

• Authors: Xi Xia, Lin Zhang, Yizhen Wang
• Journal: Microbiological research (Microbiol Res) Vol. 171 Pg. 45-51 (Feb 2015) ISSN: 1618-0623 [Electronic] Germany
• PMID: 25644952 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier GmbH. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Antimicrobial Cationic Peptides
• Cathelicidins

Topics

• Animals
• Anti-Bacterial Agents (pharmacology, therapeutic use)
• Antimicrobial Cationic Peptides (pharmacology, therapeutic use)
• Bacterial Load
• Disease Models, Animal
• Drug Stability
• Intestinal Mucosa (drug effects, microbiology, pathology, ultrastructure)
• Liver (drug effects, microbiology)
• Male
• Mice
• Salmonella Infections (drug therapy, microbiology)
• Salmonella typhimurium (drug effects)
• Spleen (drug effects, microbiology)
• Tissue Distribution
• Cathelicidins