Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: Immunization with both antigens using the heterologous strategy presented a high antibody production level while the homologous strategy immunized group showed predominantly a cellular immune response with parasite load reduction. The pcDNA-LiP0 immunized group showed increased expression ratio of IFN-γ/IL-10 and IFN-γ/TGF-β in the lymph nodes before challenge. Two months after infection hamsters immunized with the empty plasmid presented a pro-inflammatory immune response in the early stages of infection with increased expression ratio of IFN-γ/IL-10 and IFN-γ/TGF-β, whereas hamsters immunized with pcDNA-HIS presented an increase only in the ratio IFN-γ/ TGF-β. On the other hand, hamsters immunized with LiP0 did not present any increase in the IFN-γ/TGF-β and IFN-γ/IL-10 ratio independently of the immunization strategy used. Conversely, five months after infection, hamsters immunized with HIS maintained a pro-inflammatory immune response (ratio IFN-γ/ IL-10) while pcDNA-LiP0 immunized hamsters continued showing a balanced cytokine profile of pro and anti-inflammatory cytokines. Moreover we observed a significant reduction in parasite load in the spleen, liver and lymph node in this group compared with controls. CONCLUSIONS/SIGNIFICANCE:
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Authors | Lais Pereira, Melissa Abbehusen, Clarissa Teixeira, Jurema Cunha, Ivan P Nascimento, Kyioshi Fukutani, Washington dos-Santos, Aldina Barral, Camila Indiani de Oliveira, Manoel Barral-Netto, Manoel Soto, Cláudia Ida Brodskyn |
Journal | PLoS neglected tropical diseases
(PLoS Negl Trop Dis)
Vol. 9
Issue 2
Pg. e0003490
(Feb 2015)
ISSN: 1935-2735 [Electronic] United States |
PMID | 25642946
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Protozoan
- Cytokines
- Histones
- Protozoan Vaccines
- Ribosomal Proteins
- ribosomal protein P0
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Topics |
- Animals
- Antibodies, Protozoan
(blood)
- Cricetinae
- Cytokines
(biosynthesis)
- Female
- Histones
(immunology)
- Leishmania infantum
(genetics, immunology)
- Leishmaniasis, Visceral
(prevention & control)
- Male
- Mesocricetus
- Mice
- Protozoan Vaccines
(immunology)
- Ribosomal Proteins
(immunology)
- Spleen
(immunology)
- Vaccination
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