One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates.

Abstract

Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1-day pulsed-field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.

• Authors: Paulina S Kaminska, Krzysztof Fiedoruk, Dominika Jankowska, Jacques Mahillon, Karol Nowosad, Ewa Drewicka, Monika Zambrzycka, Izabela Swiecicka
• Journal: Electrophoresis (Electrophoresis) Vol. 36 Issue 7-8 Pg. 1051-4 (Apr 2015) ISSN: 1522-2683 [Electronic] Germany
• PMID: 25639850 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Depsipeptides
• cereulide

Topics

• Bacillus cereus (genetics, isolation & purification, metabolism, pathogenicity)
• Depsipeptides (metabolism)
• Electrophoresis, Gel, Pulsed-Field (methods)
• Food Contamination (analysis)
• Foodborne Diseases (microbiology)
• Humans
• Vomiting (microbiology)