Abstract |
From Chloranthus multistachys, three terpenoids - lupeol (1), henrilabdane B (2), and istanbulin A (3) were isolated. Structures of compounds were established by NMR and MS. We reported here that ISTA (3) suppressed cell invasion, but lupeol (1) and henrilabdane B (2) did not. Furthermore, ISTA significantly inhibited the ability of adhesion and migration in vitro. Next, mechanisms of ISTA-induced inhibitory effects on in vitro metastasis were investigated. Sequential treatment data revealed that ISTA dramatically inhibited EGF-induced EMT. Western blot indicated that ISTA also significantly suppressed expression of E-cadherin, vimentin, and slug. In addition, ISTA inhibited Runx2 activation and phosph-Runx2 expression. Collectively, ISTA exhibited significant inhibitory effects on in vitro metastatic potential via inducing EMT inhibition, which may be associated with inhibition of transcriptional activity of Runx2.
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Authors | Jianjiang Fu, Shan Wang, Hong Lu, Junchao Ma, Xiaoqin Ke, Ting Liu, Yongming Luo |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 22
Issue 1
Pg. 165-72
(Jan 15 2015)
ISSN: 1618-095X [Electronic] Germany |
PMID | 25636886
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier GmbH. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Core Binding Factor Alpha 1 Subunit
- Diterpenes
- Pentacyclic Triterpenes
- RUNX2 protein, human
- Sesquiterpenes
- Terpenes
- istanbulin A
- lupeol
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Breast Neoplasms
(metabolism)
- Cell Line, Tumor
- Core Binding Factor Alpha 1 Subunit
(genetics, metabolism)
- Diterpenes
(pharmacology)
- Down-Regulation
- Epithelial-Mesenchymal Transition
(drug effects)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Magnoliopsida
(chemistry)
- Molecular Structure
- Pentacyclic Triterpenes
(pharmacology)
- Plant Components, Aerial
(chemistry)
- Sesquiterpenes
(pharmacology)
- Terpenes
(pharmacology)
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