Distribution of HLA-DR alleles among Thai patients with rheumatoid arthritis.

Abstract

OBJECTIVE:

This study was performed to investigate the association between the HLA-DR series and rheumatoid arthritis (RA) in a Thai population.

METHODS:

HLA-DR subtypes were determined in 100 Thai RA patients and 99 healthy controls (HC). HLA-DR typing was performed using INNO-LiPA HLA-DRB Decoder kits (Innogenetics) and reconfirmed using MICRO SSP HLA DNA Typing kits (One Lambda) for DRB1(∗)02 and (∗)04.

RESULTS:

When compared with the HC, the RA patients had higher allele frequency (AF) of DRB1(∗)04:05 (15.00% vs 7.07%, p=0.016, pc=NS, OR=2.319, 95%CI=1.189-4.522) and DRB1(∗)10:01 (3.00% vs 0%, p=0.030, pc=NS), respectively. The DRB1(∗)09:01 was slightly higher in the RA patients, without statistical significance. The AF of the shared epitope (SE) alleles (HLA-DRB1(∗)01:01, (∗)04:01, (∗)04:05 and (∗)10:01) was significantly higher in the RA patients (18.50% vs 7.58%, p=0.002, pc=0.046, OR=2.769, 95%CI=1.466-5.231, 99%CI=1.201-6.388). The AF of HLA-DRB4(∗)01 also increased significantly more in the RA patients (40.50% vs 25.76%, p=0.002, pc=0.002, OR=1.962, 95%CI=1.282-3.003, 99%CI=1.121-3.433). The HLA-DRB3(∗)03:01 was significantly lower in the RA patients (6.00% vs 14.14%, p=0.008, pc=0.023, OR=0.388, 95%CI=0.191-0.786, 99%CI=0.153-0.982).

CONCLUSION:

In the presence of SE, the DRB1(∗)04:05 and HLA-DRB4(∗)01 were associated with RA, and the DRB3(∗)03:01 would be a protective allele against RA in a Thai population.

• Authors: Worawit Louthrenoo, Nuntana Kasitanon, Suparaporn Wangkaew, Shoji Kuwata, Fujio Takeuchi
• Journal: Human immunology (Hum Immunol) Vol. 76 Issue 2-3 Pg. 113-7 (Mar 2015) ISSN: 1879-1166 [Electronic] United States
• PMID: 25636569 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Chemical References

• Epitopes
• HLA-DR Antigens

Topics

• Adult
• Arthritis, Rheumatoid (genetics, immunology)
• Epitopes (genetics)
• Female
• Gene Frequency
• Genetic Association Studies
• Genetic Predisposition to Disease
• Genotype
• HLA-DR Antigens (genetics)
• Histocompatibility Testing
• Humans
• Male
• Middle Aged
• Polymorphism, Genetic
• Thailand