High-density lipoprotein oxidation in type 2 diabetic patients and young patients with premature myocardial infarction.

Abstract

BACKGROUND AND AIMS:

ApoA-I can undergo oxidative changes that reduce anti-atherogenic role of HDL. The aim of this study was to seek any significant differences in methionine sulfoxide (MetO) content in the ApoA-I of HDL isolated from young patients with coronary heart disease (CHD), type 2 diabetics and healthy subjects.

METHODS AND RESULTS:

We evaluated the lipid profile of 21 type 2 diabetic patients, 23 young patients with premature MI and 21 healthy volunteers; we determined in all patients the MetO content of ApoA-I in by MALDI/TOF/TOF technique. The typical MALDI spectra of the tryptic digest obtained from HDL plasma fractions all patients showed a relative abundance of peptides containing Met(112)O in ApoA-I in type 2 diabetic and CHD patients. This relative abundance is given as percentages of oxidized ApoA-I (OxApoA-I). OxApoA-I showed no significant correlations with lipoproteins in all patients studied, while a strong correlation emerged between the duration of diabetic disease and OxApoA-I levels in type 2 diabetic patients.

CONCLUSIONS:

The most remarkable finding of our study lies in the evidence it produced of an increased HDL oxidation in patients highly susceptible to CHD. Levels of MetO residues in plasma ApoA-I, measured using an accurate, specific method, should be investigated and considered in prospective future studies to assess their role in CHD.

• Authors: G Sartore, R Seraglia, S Burlina, A Bolis, R Marin, E Manzato, E Ragazzi, P Traldi, A Lapolla
• Journal: Nutrition, metabolism, and cardiovascular diseases : NMCD (Nutr Metab Cardiovasc Dis) Vol. 25 Issue 4 Pg. 418-25 (Apr 2015) ISSN: 1590-3729 [Electronic] Netherlands
• PMID: 25636381 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• APOA1 protein, human
• Apolipoprotein A-I
• Cholesterol, HDL
• Cholesterol, LDL
• Methionine
• methionine sulfoxide

Topics

• Adult
• Apolipoprotein A-I (blood)
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Cross-Sectional Studies
• Diabetes Mellitus, Type 2 (blood)
• Female
• Healthy Volunteers
• Humans
• Male
• Methionine (analogs & derivatives, metabolism)
• Middle Aged
• Myocardial Infarction (blood)
• Oxidative Stress
• Risk Factors
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization