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[Correlation of insulin-like growth factor 1 expression in placenta with DNA methylation and fetal macrosomia].

AbstractOBJECTIVE:
To explore the correlation between methylation of insulin-like growth factor 1 (IGF-1) gene promoter and its placenta-specific expression and fetal macrosoma.
METHODS:
One hundred twenty nine healthy pregnant women were recruited between April 2011 and March 2012. Baseline data were collected with self-report questionnaires. Real-time quantitative PCR was used to determine the expression of IGF-1 mRNA in the placenta. Methylation level of the IGF 1 gene was determined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
RESULTS:
The expression of IGF-1 in placenta and its methylation level showed no significant difference between macrosomic fetuses and controls. No linear correlation was found between IGF-1 mRNA expression and methylation level of IGF-1 promoter (r=0.128, P=0.295). IGF-1 promoter region in placenta showed a hypomethylation status. However, a positive correlation was found between IGF-1 expression and birth weight below 4260 g (r=0.264, P=0.022). The expression of IGF-1 mRNA was significantly higher in those with a birth weight below 4260 g, which suggested that placental IGF-1 expression may contribute to increased birth weight. In regard to fetal overgrowth, however, there seemed to be a negative correlation in which placental IGF-1 expression was downregulated to limit fetal overgrowth.
CONCLUSION:
No linear correlation was found between placental IGF-1 expression and methylation level of IGF-1 promoter with a hypomethylation status. The contribution of placental IGF-1 expression to birth weight is bidirectional. Increased expression seems to promote fetal growth, while decreased expressions may curb overgrowth, therefore control fetal growth in a relatively normal range.
AuthorsJitai Zhang, Kele Wu, Xinyun Xu, Ziwei Liu, Chong Lin, Yuhuan Wang, Hongtao Yan, Xinyun Yang
JournalZhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 32 Issue 1 Pg. 36-9 (Feb 2015) ISSN: 1003-9406 [Print] China
PMID25636096 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Messenger
  • Insulin-Like Growth Factor I
Topics
  • Birth Weight
  • DNA Methylation
  • Female
  • Fetal Macrosomia (genetics)
  • Humans
  • Infant, Newborn
  • Insulin-Like Growth Factor I (genetics)
  • Placenta (metabolism)
  • Pregnancy
  • Promoter Regions, Genetic
  • RNA, Messenger (analysis)

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