Renalase protects against contrast-induced nephropathy in Sprague-Dawley rats.

Abstract

BACKGROUND:

Contrast-induced nephropathy (CIN) is the third leading cause of hospital-acquired acute renal failure. Oxidative stress, apoptosis and inflammation play crucial roles in CIN. Renalase is a newly discovered monoamine oxidase from the kidney. We hypothesize that renalase could protect against CIN through anti-oxidation, anti-inflammation and anti-apoptosis pathways.

METHODS:

We tested our hypothesis in vivo with a rat model of Ioversol-induced CIN and in vitro. Sprague-Dawley rats were divided into 4 groups (n = 6 per group): control group, Ioversol group (rats subjected to Ioversol-induced CIN), Ioversol plus vehicle group (CIN rats pretreated with vehicle) and Ioversol plus renalase group (CIN rats pretreated with 2 mg/kg recombinant renalase). HK2 cells were treated with Ioversol or H2O2.

RESULTS:

The results showed that pretreatment with renalase attenuated the deterioration of renal function, tubular necrosis, oxidative stress, apoptosis and inflammation (P<0.05). Furthermore, renalase protected HK2 cells against the cytotoxicity of Ioversol and suppressed Caspase-3 activity, oxidative stress and apoptosis induced by H2O2.

CONCLUSION:

Recombinant renalase protected CIN in rats through anti-oxidation, anti-apoptosis and anti-inflammation mechanisms.

• Authors: Binghui Zhao, Qing Zhao, Junhui Li, Tao Xing, Feng Wang, Niansong Wang
• Journal: PloS one (PLoS One) Vol. 10 Issue 1 Pg. e0116583 ( 2015) ISSN: 1932-6203 [Electronic] United States
• PMID: 25635854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Protective Agents
• Triiodobenzoic Acids
• Creatinine
• Monoamine Oxidase
• renalase
• ioversol

Topics

• Animals
• Apoptosis (drug effects)
• Blood Urea Nitrogen
• Contrast Media (adverse effects)
• Creatinine (blood)
• Inflammation (pathology)
• Kidney (drug effects, pathology)
• Kidney Diseases (blood, chemically induced, drug therapy, pathology)
• Male
• Monoamine Oxidase (pharmacology, therapeutic use)
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology, therapeutic use)
• Rats, Sprague-Dawley
• Triiodobenzoic Acids (toxicity)