Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction.

Abstract

PURPOSE:

To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.

METHODS:

Sixteen male rats were allocated across four groups: LG--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L--Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG--Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.

RESULTS:

There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.

CONCLUSION:

Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats.

• Authors: Hugo Muscelli Alecrim, Sérgio Andurte Carvalho Duarte, Maria Eduarda Bonavides Amaral, Filipe Diógenis, Fabiana Pirani Carneiro, João Batista de Sousa
• Journal: Acta cirurgica brasileira (Acta Cir Bras) Vol. 30 Issue 1 Pg. 73-9 (Jan 2015) ISSN: 1678-2674 [Electronic] Brazil
• PMID: 25627274 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glutamine
• Bilirubin

Topics

• Animals
• Bilirubin (blood)
• Cholestasis, Extrahepatic (surgery)
• Colon (drug effects, injuries, surgery)
• Common Bile Duct (surgery)
• Dietary Supplements
• Glutamine (pharmacology)
• Jaundice, Obstructive (physiopathology)
• Ligation
• Male
• Models, Animal
• Random Allocation
• Rats, Wistar
• Reproducibility of Results
• Tensile Strength (drug effects)
• Time Factors
• Wound Healing (drug effects, physiology)