Abstract |
Staphylococcal enterotoxin B (SEB) is a major virulence factor for staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T lymphocytic population, releasing proinflammatory cytokines. Blocking SEB-initiated toxicity may be an effective strategy for treating TSS. Using a process known as systematic evolution of ligands by exponential enrichment (SELEX), we identified an aptamer that can antagonize SEB with nanomolar binding affinity (Kd = 64 nM). The aptamer antagonist effectively inhibits SEB-mediated proliferation and cytokine secretion in human peripheral blood mononuclear cells. Moreover, a PEGylated aptamer antagonist significantly reduced mortality in a "double-hit" mouse model of SEB-induced TSS, established via sensitization with d- galactosamine followed by SEB challenge. Therefore, our novel aptamer antagonist may offer potential therapeutic efficacy against SEB-mediated TSS.
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Authors | Kaiyu Wang, Longjie Gan, Li Jiang, Xianhui Zhang, Xiangyue Yang, Min Chen, Xiaopeng Lan |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 59
Issue 4
Pg. 2072-7
(Apr 2015)
ISSN: 1098-6596 [Electronic] United States |
PMID | 25624325
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015, American Society for Microbiology. All Rights Reserved. |
Chemical References |
- Cytokines
- Enterotoxins
- enterotoxin B, staphylococcal
- Galactosamine
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Topics |
- Animals
- Cell Proliferation
(drug effects)
- Cloning, Molecular
- Computational Biology
- Cytokines
(biosynthesis)
- Drug Evaluation, Preclinical
- Enterotoxins
(antagonists & inhibitors)
- Female
- Galactosamine
(pharmacology)
- Humans
- Mice
- Mice, Inbred BALB C
- Monocytes
(drug effects)
- SELEX Aptamer Technique
- Shock, Septic
(drug therapy, microbiology)
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