Auraptene has the inhibitory property on murine T lymphocyte activation.

Abstract

Auraptene, a citrus fruit-derived coumarin, has been reported to exert valuable pharmacological properties as anti-tumor, anti-inflammatory, and anti-oxidant agent. However, little is known about auraptene on immune responses. In this study, we conducted an investigation to evaluate auraptene as an anti-T lymphocyte proliferation agent using CD3/CD28-activated lymphocytes isolated from C57BL/6 mice. We found that administration of auraptene inhibited CD3/CD28-activated lymphocyte proliferation in a dose dependent manner, but the inhibition at a wide range of doses used in this study did not induce cytotoxicity or apoptosis. In addition, auraptene dose dependently decreased the CD3/CD28-activated T lymphocyte secreting T helper (Th)1 cytokines (interleukin (IL)-2 and interferon (IFN)-γ); whereas, auraptene could decrease Th2 cytokine (IL-4) at a higher level (40µM) but had not at lower levels (10 and 20µM). Further mechanistic study demonstrated that auraptene doses dependently suppressed T cell early and middle/late activation marker CD69 and CD25 expression, respectively. Finally, auraptene could suppress cell cycle progression which contributes to inhibiting T cell proliferation and cell division. These findings indicate that auraptene exhibits anti-inflammatory properties via inhibiting T cell proliferation and their inflammatory cytokine secretion that may mediate the interaction between T cells and autoimmune disorders, suggesting that auraptene is a potential food-derived compound with a benefit to those with abnormally over-activation T cell mediated response and chronic inflammation such as autoimmune and inflammatory diseases.

• Authors: Xinli Niu, Zhihong Huang, Lin Zhang, Xuequn Ren, Junpeng Wang
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 750 Pg. 8-13 (Mar 05 2015) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 25620131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• CD28 Antigens
• CD3 Complex
• Coumarins
• Cytokines
• Lipopolysaccharides
• aurapten

Topics

• Animals
• CD28 Antigens (metabolism)
• CD3 Complex (metabolism)
• Cell Division (drug effects)
• Cell Proliferation (drug effects)
• Coumarins (pharmacology)
• Cytokines (biosynthesis, metabolism)
• Female
• Lipopolysaccharides (adverse effects)
• Lymphocyte Activation (drug effects)
• Mice
• Mice, Inbred C57BL
• T-Lymphocytes (cytology, drug effects, immunology, metabolism)