Kinetics of 3H-thymidine label in rat liver regenerating after partial hepatectomy and after damage with carbon tetrachloride or silica.

Rat liver DNA was labelled with [methyl-3H] thymidine after partial hepatectomy, carbon tetrachloride poisoning, or an intravenous injection of silica dust. Changes in DNA labelling were studied for 4 weeks after the single pulse. Total radioactivity incorporated into liver DNA after partial hepatectomy and after carbon tetrachloride administration remained on the same level when compared with that found after 1 h. DNA activity in liver of untreated rats and of rats treated with silica decreased by about 50% within the first 2 weeks and then remained on this level for the rest of the studied period. These differences may reflect the fact that hepatocytes that have a long life span are preferentially labelled in partially hepatectomized and CCl4-treated rats, while liver macrophages with a short half-life take up a large part of the label in intact rats and in rats treated with silica.
AuthorsJ Kanta, V Chmelar
JournalSborník vědeckých prací Lékařské fakulty Karlovy university v Hradci Králové (Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove) Vol. 32 Issue 4 Pg. 395-401 ( 1989) ISSN: 0049-5514 [Print] CZECHOSLOVAKIA
PMID2561578 (Publication Type: Journal Article)
Chemical References
  • Dust
  • Silicon Dioxide
  • DNA
  • Carbon Tetrachloride
  • Thymidine
  • Animals
  • Carbon Tetrachloride (toxicity)
  • DNA (drug effects, metabolism)
  • Dust (adverse effects)
  • Female
  • Hepatectomy
  • Liver (drug effects, metabolism)
  • Liver Regeneration
  • Rats
  • Rats, Inbred Strains
  • Silicon Dioxide (adverse effects)
  • Thymidine (metabolism)

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