High-resolution melting analysis of MED12 mutations in uterine leiomyomas in Chinese patients.

Abstract

OBJECTIVES:

Somatic mutations in mediator complex subunit 12 (MED12) have emerged as a critical genetic change in the development of uterine leiomyomas. Studies, however, have focused largely on cohorts consisting of Caucasian patients. In this study, uterine leiomyomas from Chinese patients were examined for MED12 mutations. In addition, polymerase chain reaction (PCR)-based high-resolution melting analysis (HRMA) was compared with direct sequencing as a potentially more sensitive method for the detection of MED12 mutations.

METHODS:

Tissue samples with the pathologies of uterine leiomyoma (n=181) and other endometrial diseases (n=157) were collected from Chinese patients at the Taizhou People's Hospital and Taizhou Polytechnic College (Taizhou City, China). Genomic DNA was prepared from all samples. Both PCR-based HRMA and PCR-based direct sequencing were used to detect MED12 mutations.

RESULTS:

PCR-based HRMA and direct sequencing revealed MED12 mutations in 95/181 (52.5%) and 93/181 (51.4%) uterine leiomyomas, respectively. Nearly half of these mutations (46/93) were found in a single codon, codon 131. The coincidence rate between the two methods was 98.9% (179/181) so that no statistically significant difference was evident in the application of the methodologies (χ(2)=0.011, p=0.916). In addition, MED12 mutations were identified in 1/157 (4.17%) case of other endometrial pathologies by both methods.

CONCLUSIONS:

MED12 mutations were closely associated with the development of uterine leiomyomas, as opposed to other uterine pathologies in Chinese patients, and PCR-based HRMA was found to be a reliable method for the detection of MED12 mutations.

• Authors: Hua Wang, Jun Ye, Hua Qian, Ruifang Zhou, Jun Jiang, Lihua Ye
• Journal: Genetic testing and molecular biomarkers (Genet Test Mol Biomarkers) Vol. 19 Issue 3 Pg. 162-6 (Mar 2015) ISSN: 1945-0257 [Electronic] United States
• PMID: 25615570 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MED12 protein, human
• Mediator Complex

Topics

• Adult
• Aged
• Asian People (genetics)
• Female
• Genetic Predisposition to Disease
• Humans
• Leiomyoma (genetics)
• Mediator Complex (genetics)
• Middle Aged
• Mutation
• Polymerase Chain Reaction (methods)
• Sequence Analysis, DNA (methods)
• Uterine Neoplasms (genetics)