Oleuropein suppresses LPS-induced inflammatory responses in RAW 264.7 cell and zebrafish.

Abstract

Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent.

• Authors: Su-Jung Ryu, Hyeon-Son Choi, Kye-Yoon Yoon, Ok-Hwan Lee, Kui-Jin Kim, Boo-Yong Lee
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 63 Issue 7 Pg. 2098-105 (Feb 25 2015) ISSN: 1520-5118 [Electronic] United States
• PMID: 25613688 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Interleukin-6
• Iridoid Glucosides
• Iridoids
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• oleuropein
• Cyclooxygenase 2

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Cyclooxygenase 2 (genetics, immunology)
• Inflammation (drug therapy, genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Iridoid Glucosides
• Iridoids (administration & dosage)
• Lipopolysaccharides (adverse effects, immunology)
• Macrophages (drug effects, immunology)
• Mice
• NF-kappa B (genetics, immunology)
• Olea (chemistry)
• Plant Extracts (administration & dosage)
• Plant Leaves (chemistry)
• Zebrafish