Abstract |
To prevent the progression of the disease, we treated leukemic or preleukemic cows with (1) adriamycin (ADM) entrapped in liposomes conjugated with monoclonal antibody, c143, against tumor-associated antigens (TAA) of bovine leukemic cells and (2) an immunotherapy using an immunopotentiator consisting of the cell wall skeleton of Nocardia rubra ( N-CWS). Five leukemic or preleukemic cows with TAA-positive peripheral blood lymphocytes (PBL) received four injections of ADM alone (0.4 mg/kg body weight) or c143-conjugated liposomes containing the same dose of ADM (L-ADM-c143) through the jugular vein at about 4-day intervals. In three animals treated with L-ADM-c143, the TAA-positive cells gradually decreased with treatment and finally two animals became TAA-negative during a 6-week period and a 14-week period after treatment, respectively. About 6 weeks later, however, TAA-positive cells gradually increased. In the control, two animals treated with ADM alone showed no decrease of TAA-positive cells. Five TAA-positive animals with enlarged subcutaneous lymphatic nodules, each nodule estimated to be from 1 to 4 cm3 in size, were treated by injection of N-CWS into the tumors. Complete regression of tumor was observed in seven out of ten tumors treated in five animals. Decrease of TAA-positive cells was also observed in PBL for all five treated animals. In one animal, the TAA-positive cells remained low for at least 280 days after treatment. This study documents that ADM treatment and intralesionally administered N-CWS are effective in the treatment of bovine leukosis.
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Authors | M Onuma, Y Yasutomi, M Yamamoto |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 22
Issue 3
Pg. 245-54
(Oct 1989)
ISSN: 0165-2427 [Print] Netherlands |
PMID | 2560856
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Monoclonal
- Antigens, Viral, Tumor
- Drug Carriers
- Liposomes
- Doxorubicin
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Animals
- Antibodies, Monoclonal
(administration & dosage)
- Antigens, Viral, Tumor
- Cattle
- Cattle Diseases
(drug therapy, immunology, therapy)
- Combined Modality Therapy
- Doxorubicin
(administration & dosage)
- Drug Carriers
- Immunotherapy
- Leukemia
(drug therapy, therapy, veterinary)
- Leukemia Virus, Bovine
- Liposomes
- Preleukemia
(drug therapy, therapy, veterinary)
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