[Proliferation inhibition and apoptosis induction of Juglone on human cervical cancer Caski cells].

Abstract

OBJECTIVE:

To explore the effects of Juglone on proliferation and apoptosis of human cervical cancer Caski cells, and to further study the related mechanism of cell apoptosis.

METHODS:

Cultured Caski cells were incubated with 20, 40, 60, 80 and 100 μmol/L juglone for 24 h. The proliferation of Caski cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The cell apoptosis were detected by transmission electron microscope. The expression of Bcl-2 and Bax were detected by Western blot.

RESULTS:

MTT results showed that in different doses of juglone groups, the Caski cell growth was greatly inhibited (P < 0.05, P < 0.01) and showed dose dependent when compared with control group except 20 μmol/L. The IC50 of juglone was 42.4 μmol/L. After treatment on Caski cells with 40 μmol/L juglone, typical apoptosis characteristics was observed by transmission electronmicro scope. The expression of Bcl-2 was decreased while the expression of Bax was increased significantly when compared with control group (P < 0.05).

CONCLUSION:

Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.

• Authors: Wei Zhang, Yan Li, Jun Luo, Xingyu Zhao, Junjie Xu, Wenhe Zhu, Yanxia Jiang, Fang Fang
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 43 Issue 6 Pg. 959-61, 971 (Nov 2014) ISSN: 1000-8020 [Print] China
• PMID: 25603606 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• BAX protein, human
• Naphthoquinones
• bcl-2-Associated X Protein
• juglone

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Cycle (physiology)
• Cell Proliferation (drug effects)
• Female
• Humans
• Microscopy, Electron, Transmission
• Naphthoquinones (pharmacology)
• Uterine Cervical Neoplasms (drug therapy, metabolism)
• bcl-2-Associated X Protein (metabolism)