[Function study of non-syndromic deafness associated mitochondrial 12S rRNA A839G mutation].

Abstract

OBJECTIVE:

To investigate the correlation between nonsyndromic deafness and mitochondrial 12s rRNA A839G mutation.

METHODS:

According to the clinical manifestations of mitochondrial DNA sequencing and analysis to find and determine family containing mitochondrial 12s rRNA A839G mutation. Harvested its family members blood and transferred their lymphocytes into lymphoblastoid cell lines, followed by cells cultured, cell doubling experiment, susceptibility testing, cellular oxygen consumption rate experiment, ROS and mitochondrial membrane potential experimental tests were progressed to explore the correlation between the A839G mutation and nonsyndromic deafness.

RESULTS:

The mitochondrial 12s rRNA A839G mutation pedigrees were determined through the full sequence detections of the Mitochondrial DNA, further phylogenetic analysis showed that 839 point conservative index (CI) up to 78.6%; in RPMI-galactose medium containing A839G gene mutant cell line, the doubling time was significantly longer than the control group, and the difference was significant (P = 0.033). The effect to cell lines containing the A839G mutation of aminoglycoside drugs was not obvious. When compared with the control group, cell lines containing the A839G mutation significantly reduced cellular oxygen consumption rate(P = 0.033); compared with the control group, the ROS levels of cell lines containing the A839G mutation appeared more substantial elevated with significan difference (P < 0.01). The mitochondrial membrane potential of cells of experimental group was significantly reduced than the control group.

CONCLUSION:

The present study proved that the mitochondria 12s rRNA A839G mutations affect the function of the mitochondrial respiratory chain at the cell level, which might reduce the growth rate of the mutant cell lines, result in hearing.

• Authors: Xiao Yu, Zheyun He, Haijie Xiang, Jing Zheng, Benyu Nan, Binjiao Zheng, Jinjian Gao, Saiyu Huang, Minxin Guan, Bobei Chen
• Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 49 Issue 11 Pg. 908-15 (Nov 2014) ISSN: 1673-0860 [Print] China
• PMID: 25598369 (Publication Type: Journal Article)

Chemical References

• Aminoglycosides
• DNA, Mitochondrial
• RNA, Ribosomal
• RNA, ribosomal, 12S
• Galactose

Topics

• Aminoglycosides
• Cell Line
• DNA, Mitochondrial
• Deafness (genetics)
• Galactose
• Hearing Tests
• Mitochondria
• Mutation
• Pedigree
• Phylogeny
• RNA, Ribosomal (genetics)