Changes in B cell and T cell subsets in bovine leukaemia virus-infected cattle.

Abstract

Direct immunofluorescence and fluorescence-activated cell sorter techniques were used for the detection of surface immunoglobulin positive (SIg+) cells in peripheral blood lymphocytes (PBL's) of bovine leukaemia virus (BLV) infected cattle with or without persistent lymphocytosis (PL+, PL-) and in BLV-free cattle. The percentage of SIg+ cells was more than twice as high in BLV+PL+ cattle than in BLV-free and BLV+PL- cattle. Bovine T cells, and T cell subsets were identified indirectly by the same techniques using three monoclonal antibodies (MAb's) specific for all T cells (IL-A43), T helper (BoT4) cells (IL-A12) and T cytotoxic (BoT8) cells (IL-A17). The major histocompatibility complex (MHC) determinants of both class II (BoT4) and class I (BoT8) as well as all T cells were significantly reduced in BLV+PL+ compared to BLV-free cattle. The actual decrease in the BoT8 cell subset or the dilution effect that would change effector:target cell ratio suggests that a resultant decrease in cytotoxic activity in BLV+PL+ cattle may play an important role in the progress of BLV infection in cattle.

• Authors: M H Gatei, R B Brandon, H M Naif, M W McLennan, R C Daniel, M F Lavin
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 23 Issue 1-2 Pg. 139-47 (Nov 30 1989) ISSN: 0165-2427 [Print] Netherlands
• PMID: 2559534 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal

Topics

• Animals
• Antibodies, Monoclonal
• B-Lymphocytes (immunology)
• Cattle
• Cattle Diseases (blood, immunology)
• Cytotoxicity, Immunologic
• Leukemia (blood, immunology, veterinary)
• Leukemia Virus, Bovine
• Leukocyte Count
• T-Lymphocytes (immunology)