Alginate-calcium microsphere loaded with thrombin: a new composite biomaterial for hemostatic embolization.

Abstract

To date, transcatheter arterial embolization (TAE) has become a standard treatment to control intracavitary bleeding as an alternative to surgery. Due to excellent biocompatibility and no residual in vivo, biodegradable materials are preferred in TAE. However, gelfoam is the only commercially available biodegradable embolic material used to treat blunt trauma of solid abdominal viscera until now, and controversial on its stability and reliability never stopped in the past five decades. In this study, a new biodegradable macromolecule material (thrombin-loaded alginate-calcium microspheres, TACMs) was prepared using electrostatic droplet techniques and a special method was developed for hemostatic embolization. Thrombin was successfully loaded into microspheres with high encapsulation efficiency and drug loading capacity. A burst release of TACMs was observed at early stage and sustained release later on, with the activity of thrombin preserved well. The strength of TACMs mixed thrombus, which was used as embolic agent, increased in a dose-dependent manner after TACMs were added. In addition, the TACMs were verified to be of no cytotoxicity and systemic toxicity, and biodegradable in vivo. Finally, the results of preliminary applications revealed that the TACMs could serve as an effective and promising embolic material for blunt trauma and hemorrhage of solid abdominal viscera.

• Authors: Jing-jing Rong, Ming Liang, Feng-qi Xuan, Jing-yang Sun, Li-jun Zhao, Hui-zhen Zhen, Xiao-xiang Tian, Dan Liu, Quan-yu Zhang, Cheng-fei Peng, Tian-ming Yao, Fei Li, Xiao-zeng Wang, Ya-ling Han, Wei-ting Yu
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 75 Pg. 479-88 (Apr 2015) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 25583022 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Alginates
• Biocompatible Materials
• Hemostatics
• Hexuronic Acids
• Glucuronic Acid
• Thrombin
• Calcium

Topics

• Alginates (chemistry)
• Animals
• Biocompatible Materials (pharmacology)
• Blood Coagulation (drug effects)
• Body Weight (drug effects)
• Calcium (chemistry)
• Cell Death (drug effects)
• Cell Line
• Disease Models, Animal
• Drug Liberation
• Embolization, Therapeutic
• Glucuronic Acid (chemistry)
• Hemostatics (pharmacology)
• Hexuronic Acids (chemistry)
• Kidney (drug effects, pathology)
• Male
• Mice, Inbred C57BL
• Microscopy, Electron, Scanning
• Microspheres
• Organ Specificity (drug effects)
• Particle Size
• Rabbits
• Rats, Sprague-Dawley
• Renal Artery (drug effects, pathology)
• Subcutaneous Tissue (drug effects)
• Thrombin (pharmacology)
• Toxicity Tests