Physico-chemical characterization and pharmacological evaluation of sulfated polysaccharides from three species of Mediterranean brown algae of the genus Cystoseira.

Abstract

BACKGROUND:

Seaweed polysaccharides are highly active natural substances having valuable applications. The present study was conducted to characterize the physico-chemical properties of sulphated polysaccharides from three Mediterranean brown seaweeds (Cystoseira sedoides, Cystoseira compressa and Cystoseira crinita) and to evaluate their anti-radical, anti-inflammatory and gastroprotective activities.

METHODS:

The different rates of neutral sugars, uronic acids, L-fucose and sulphate content were determined by colorimetric techniques. The different macromolecular characteristics of isolated fucoidans were identified by size exclusion chromatography equipped with a triple detection: multiangle light scattering, viscometer and differential refractive index detectors, (SEC/MALS/VD/DRI). Anti-inflammatory activity was evaluated, using the carrageenan-induced rat paw edema test in comparison to the references drugs Acetylsalicylate of Lysine and Diclofenac. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats and to examine the antioxidant effect of fucoidans in the three species, the free radical scavenging activity was determined using 1,1-diphenyl-2-picrylhydrazyl.

RESULTS:

The pharmacological evaluation of the isolated fucoidans for their anti-inflammatory, and their gastroprotective effect established that these products from C. sedoides, C. compressa and C. crinita exhibited a significant anti-inflammatory activity at a dose of 50 mg/kg, i.p; the percentages of inhibition of the oedema were 51%, 57% and 58% respectively. And, at the same dose, these fucoidans from C. sedoides and C. compressa showed a significant decrease of the intensity of gastric mucosal damages compared to a control group by 68%, whereas, the fucoidan from C. crinita produced a less gastroprotective effect. Furthermore, the isolated fucoidans exhibited a radical scavenging activity.

CONCLUSION:

The comparative study of fucoidans isolated from three species of the genus Cystoseira showed that they have similar chemicals properties and relatives anti-radical, anti-inflammatory and gastroprotective activities which are found to be promising.

• Authors: Hiba Hadj Ammar, Sirine Lajili, Rafik Ben Said, Didier Le Cerf, Abderrahman Bouraoui, Hatem Majdoub
• Journal: Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences (Daru) Vol. 23 Pg. 1 (Jan 13 2015) ISSN: 2008-2231 [Electronic] Switzerland
• PMID: 25582169 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Polysaccharides
• Ethanol
• Carrageenan
• fucoidan
• Hydrochloric Acid

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, chemistry, isolation & purification)
• Antioxidants (administration & dosage, chemistry, isolation & purification)
• Carrageenan
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Edema (chemically induced, drug therapy)
• Ethanol
• Female
• Hydrochloric Acid
• Male
• Phaeophyta (chemistry, classification)
• Polysaccharides (administration & dosage, chemistry, isolation & purification)
• Rats
• Rats, Wistar
• Stomach Ulcer (chemically induced, prevention & control)