HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells.

Abstract
Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.
AuthorsJie Qiao, Shu-Jian Cui, Lei-Lei Xu, Si-Jie Chen, Jun Yao, Ying-Hua Jiang, Gang Peng, Cai-Yun Fang, Peng-Yuan Yang, Feng Liu
JournalOncotarget (Oncotarget) Vol. 6 Issue 2 Pg. 1171-89 (Jan 20 2015) ISSN: 1949-2553 [Electronic] United States
PMID25577646 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Filamins
  • Proteome
Topics
  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Line, Tumor
  • Cell Movement (genetics)
  • Cell Proliferation (genetics)
  • Chromatography, Liquid
  • Female
  • Filamins (genetics, metabolism)
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Neoplasms (genetics, metabolism, pathology)
  • Proteome (genetics, metabolism)
  • Proteomics (methods)
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms (genetics, metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: