microRNA-377 suppresses the proliferation of human osteosarcoma MG-63 cells by targeting CDK6.

Abstract

MicroRNAs (miRNAs) are essential to the progression of osteosarcoma. Previous research using osteosarcoma samples confirmed that miR-377 expression is less than that observed in normal human osteoblast expression. These data suggest a role for miR-377 in osteosarcoma that warrants investigation. To address this concept, we measured miR-377 expression in two cell models, and we also observed that miR-377 was less expressed in osteosarcoma MG-63 cells compared to the hFOB1.19 human fetal osteoblastic cell line. Moreover, miR-377 overexpression reduced cell proliferation and suppressed invasion of MG-63 cells but had no effect on MG-63 cell apoptosis. Because cyclin-dependent kinase 6 (CDK6) may be a potential target of miR-377 in osteosarcoma cells, we overexpressed CDK6 and observed that overexpression attenuated tumor suppressive effects of miR-377 on cell proliferation. Our data suggest that miR-377 can suppress proliferation in MG-63 cells in part by targeting CDK6.

• Authors: Liang Wang, Jianli Shao, Xia Zhang, Meng Xu, Jianfu Zhao
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 36 Issue 5 Pg. 3911-7 (May 2015) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 25577249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN377 microRNA, human
• MicroRNAs
• Cyclin-Dependent Kinase 6

Topics

• Apoptosis (genetics)
• Bone Neoplasms (genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cyclin-Dependent Kinase 6 (biosynthesis, genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs (biosynthesis, genetics)
• Neoplasm Invasiveness (genetics)
• Osteosarcoma (genetics, pathology)