Abstract | BACKGROUND AND PURPOSE: MATERIALS AND METHODS: Three HNSCC cell lines (SCC25, CAL27 and FaDu) were treated with increasing doses of cafestol. Then combination experiments with cisplatin and irradiation were carried out. Drug interactions and possible synergy were calculated using the combination index analysis. Clonogenic assays were performed after irradiation with 2, 4, 6 and 8 Gy, respectively, and the rate of apoptosis was measured with flow cytometry. RESULTS: Treatment of HNSCC cells with cafestol leads to a dose-dependent reduction of cell viability and to induction of apoptosis. Combination with irradiation shows a reduction of clonogenic survival compared to each treatment method alone. In two of the cell lines a significant additive effect was observed. CONCLUSION:
Cafestol is a naturally occurring effective compound with growth-inhibiting properties in head and neck cancer cells. Moreover, it leads to a significant inhibition of colony formation.
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Authors | Ulana Kotowski, Gregor Heiduschka, Rudolf Seemann, Julia Eckl-Dorna, Rainer Schmid, Veronika Kranebitter, Isabella Stanisz, Markus Brunner, Claudia Lill, Dietmar Thurnher |
Journal | Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]
(Strahlenther Onkol)
Vol. 191
Issue 6
Pg. 511-7
(Jun 2015)
ISSN: 1439-099X [Electronic] Germany |
PMID | 25575980
(Publication Type: Journal Article)
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Chemical References |
- Coffee
- Diterpenes
- cafestol
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Topics |
- Carcinoma, Squamous Cell
(pathology, physiopathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects, radiation effects)
- Chemoradiotherapy
(methods)
- Coffee
(chemistry)
- Diterpenes
(administration & dosage)
- Head and Neck Neoplasms
(pathology, physiopathology)
- Humans
- Squamous Cell Carcinoma of Head and Neck
- Treatment Outcome
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