Low concentrations of cylindrospermopsin induce increases of reactive oxygen species levels, metabolism and proliferation in human hepatoma cells (HepG2).

Human hepatoma cells (HepG2) were exposed to purified cylindrospermopsin (CYN), a potent toxicant for eukaryotic cells produced by several cyanobacteria. CYN was not toxic at concentrations up to 10 μgl(-1), leading to increased viability and metabolism in cells cultured with 10% fetal bovine serum (FBS). Reduction of FBS concentration to 2% and induction of cytochrome P450 (CYP) isoforms were performed in order to make xenobiotic-metabolizing capacity of HepG2 cells closest to that of 'normal' cells. HepG2 cells proliferated less after CYPs-induction, and this induction has lead to similar results of non-induced cells, except for few individual parameters such lipid peroxidation. Foremost, low concentrations of CYN (below or equal 10 μgl(-1)) have induced HepG2 cells proliferation and metabolism increase, which was not expected.
AuthorsSamuel Liebel, Ciro Alberto de Oliveira Ribeiro, Valéria Freitas de Magalhães, Rodrigo de Cássio da Silva, Stéfani Cibele Rossi, Marco Antônio Ferreira Randi, Francisco Filipak Neto
JournalToxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 29 Issue 3 Pg. 479-88 (Apr 2015) ISSN: 1879-3177 [Electronic] England
PMID25575781 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier Ltd. All rights reserved.

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