Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion.

Abstract

PURPOSE:

The present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.

METHODS:

Wound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.

RESULTS:

We found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.

CONCLUSIONS:

These findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.

• Authors: X Song, L Hua, Y Xu, Z Fang, Y Wang, J Gao, Q Shi, X Zhou, R Yu
• Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (Clin Transl Oncol) Vol. 17 Issue 6 Pg. 477-85 (Jun 2015) ISSN: 1699-3055 [Electronic] Italy
• PMID: 25573158 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Small Interfering
• Ralb protein, human
• Alkyl and Aryl Transferases
• geranylgeranyltransferase type-I
• ral GTP-Binding Proteins

Topics

• Alkyl and Aryl Transferases (metabolism)
• Blotting, Western
• Cell Line, Tumor
• Cell Movement (physiology)
• Glioma (pathology)
• Humans
• Neoplasm Invasiveness (pathology)
• RNA, Small Interfering
• Transfection
• ral GTP-Binding Proteins (metabolism)