Abstract |
Traditionally, synthetic peptide vaccines for infectious diseases and cancer require adjuvants to achieve optimal immunogenicity. Here we describe a novel method of peptide modification using a fluorocarbon chain which can substantially increase peptide-specific cellular immune responses in the absence of adjuvant. We demonstrate that fluorocarbon-modified peptides (fluoropeptides) derived from HIV, influenza and hepatitis C virus can significantly increase interferon gamma ELISpot responses against cytotoxic and T-helper epitopes compared to unmodified peptides or lipopeptides in mice. Increases in both T-helper1 and T-helper2 cytokines are observed. Fluoropeptides show enhanced ability of the antigen to persist at the site of administration and persistence is associated with a prolonged and elevated immune response. Additionally we demonstrate that fluoropeptides have increased proteolytic resistance thereby potentially supporting their increased half-life in vivo. Fluorocarbon-modification of peptides provides a valuable tool for increasing cellular immunogenicity of vaccines for infectious diseases and cancer without requirement for traditional adjuvants.
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Authors | James N Francis, Jean-François Thaburet, Dominique Bonnet, Philip J Sizer, Carlton B Brown, Bertrand Georges |
Journal | Vaccine
(Vaccine)
Vol. 33
Issue 8
Pg. 1071-6
(Feb 18 2015)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 25573036
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Adjuvants, Immunologic
- Cytokines
- Fluorocarbons
- Micelles
- Peptides
- Vaccines, Subunit
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Topics |
- Adjuvants, Immunologic
- Amino Acid Sequence
- Animals
- Cytokines
(biosynthesis)
- Female
- Fluorocarbons
- Immunity, Cellular
- Immunization
- Lymphocyte Activation
(immunology)
- Mice
- Micelles
- Molecular Sequence Data
- Peptides
(chemistry, immunology)
- Proteolysis
- Vaccines, Subunit
(administration & dosage, immunology)
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