Protective effect of δ-amyrone against ethanol-induced gastric ulcer in mice.

Abstract

The purpose of this study is to examine the protective effect of δ-amyrone on ethanol-induced gastric ulcer in mice. The mice intragastric administration 75% (0.5 mL/100g) ethanol was pretreated with δ-amyrone (4 and 8 mg/kg) and cimetidine (100 mg/kg) or vehicles in different experimental groups for a continuous three-day, and animals were euthanized 3h after ethanol ingestion. The gastric lesions were significantly attenuated by δ-amyrone (4 and 8 mg/kg) as compared to the ulcer control group. Pre-treatment with δ-amyrone prevented the myeloperoxidase (MPO) activity, production of nitric oxide (NO) in serum, expression of inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-κB) p65 protein expression. Analysis of cytokines in gastric tissue and serum of ethanol-induced mice showed the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were decreased by δ-amyrone in response to NF-κB p65. These results suggested that δ-amyrone exerts its protective effect on experimental gastric ulcer by inhibiting NF-κB signaling pathways, which subsequently reduces overproduction of the inducible enzymes iNOS and suppresses the release of the inflammatory factors TNF-α, IL-6 and NO. Thus, δ-amyrone shows promise as a therapeutic agent in experimental gastric ulcer.

• Authors: Weifeng Li, Huan Yao, Xiaofeng Niu, Yu Wang, Hailin Zhang, Huani Li, Qingli Mu
• Journal: Immunobiology (Immunobiology) Vol. 220 Issue 6 Pg. 798-806 (Jun 2015) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 25572867 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier GmbH. All rights reserved.

Chemical References

• Cytokines
• Protective Agents
• Transcription Factor RelA
• Triterpenes
• amyrone
• Nitric Oxide
• Ethanol
• Peroxidase
• Nitric Oxide Synthase Type II

Topics

• Animals
• Cytokines (biosynthesis, blood)
• Disease Models, Animal
• Ethanol (adverse effects)
• Gastric Acidity Determination
• Gastric Mucosa (drug effects, metabolism, pathology)
• Immunohistochemistry
• Male
• Mice
• Mucus (metabolism)
• Nitric Oxide (blood, metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Peroxidase (metabolism)
• Protective Agents (administration & dosage, pharmacology)
• Stomach Ulcer (chemically induced, drug therapy, metabolism, pathology)
• Transcription Factor RelA (metabolism)
• Triterpenes (administration & dosage, pharmacology)