[Regulation of Thl/Th2 cells by T cell-mediated transcription factor in rats with chronic obstructive pulmonary disease].

Abstract

OBJECTIVE:

To determine T box expressed in T cells (T bet), GATA binding protein-3 (GATA 3), and retinoid-related orphan nuclear receptor gammat (RORgammat) in rats with chronic obstructive pulmonary disease (COPD).

METHODS:

Thirty rats were randomly divided into a control and a COPD group. The COPD model was established through smoking and lipopolysaccharide (LPS) tracheal instillation. Pulmonary function of the rats was measured 28 d after the establishment of the COPD model by a spirometer. Enzyme linked immunosorbent assay was performed to detect serum γ interferon (IFN-γ), interleukin (IL)-4, and IL-17. The expressions of T-bet, GATA-3, and RORgammat protein in lung tissues were determined by Western blot.

RESULTS:

Compared with the controls, the COPD rats had decreased pulmonary function and expression of serum IL-4, and increased INF-γ, IL- 17, Th1/Th2, T-bet, T bet /GATA-3, and RORgammat protein (P<0. 05). Forced expiratory volume in 0. 3 seconds (FEV 0.3) was negatively correlated with INF γ and T-bet/GATA-3. Forced vital capacity (FVC) was positively correlated with IL 4. FEV0.3/FVC was negatively correlated with Thl/Th2, T-bet and T-bet/GATA-3. Peak expiratory flow (PEF) was negatively correlated with IL-17, T bet, and RORgammat (P<0. 05). Thl/Th2 was positively correlated with T bet/GATA-3. IL-17 was positively correlated with RORgammat. T bet/GATA-3 was positively correlated with RORgammat (P<0. 05).

CONCLUSION:

Imbalanced regulation of T bet / GATA 3 and RORgammat on Th1/Th2 and Th17 cells is associated with the occurrence of COPD.

• Authors: Cheng-yangi Wang, Xiang-guo Liu, Chuan-bo Wang, Qiao Rue Ji, Li Fang, Ze Li Ze geng
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 45 Issue 6 Pg. 941-5 (Nov 2014) ISSN: 1672-173X [Print] China
• PMID: 25571720 (Publication Type: Journal Article)

Chemical References

• GATA3 Transcription Factor
• Interleukin-17
• Lipopolysaccharides
• Nuclear Receptor Subfamily 1, Group F, Member 3
• RNA, Messenger
• Rorc protein, rat
• T-Box Domain Proteins
• T-box transcription factor TBX21
• Interleukin-4
• Interferon-gamma

Topics

• Animals
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• GATA3 Transcription Factor (metabolism)
• Interferon-gamma (blood)
• Interleukin-17 (blood)
• Interleukin-4 (blood)
• Lipopolysaccharides
• Lung (physiopathology)
• Nuclear Receptor Subfamily 1, Group F, Member 3 (metabolism)
• Pulmonary Disease, Chronic Obstructive (immunology)
• RNA, Messenger
• Rats
• T-Box Domain Proteins (metabolism)
• Th1-Th2 Balance
• Th17 Cells (immunology)