[The quantity and distribution of specific glycoproteins in gastric carcinoma].

Abstract

42 tissue samples of gastric carcinoma were stained by HE, PAS and AB, and treated by Immuno-histochemistry ABC with 7 kinds of Lectin to study the meaning of the changes of glycoprotein in their different types and different sites of the gastric mucosa. The binding indexes of Dolichos biflorus agglutinin (DBA) (6.81) and Soybean agglutinin (SBA) (7.02) were strong (P less than 0.01) in mucus carcinoma. In undifferentiated glandular carcinoma, Wheat germ agglutinin (WGA) was high (6.98, P less than 0.05). WGA staining in the tumor of invaded nerves or vessels was strong. The reactions of Ricinus communis agglutinin (RCA) and WGA in most tumors were stronger than those of the other Lectins. Some positive areas of Lectin binding were negatively presented after treating with PAS and AB methods. The results indicated that Lectin binding was more sensitive than classical histochemical methods. The changes of DBA and SBA showed increased N-acetyl-D-galactosamine in mucus carcinoma. The changes of WGA suggested that the increased sialic acid reaction was related to the differentiation, invasion and metastasis of tumor.

• Authors: Z Y Chen
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 69 Issue 7 Pg. 362-4, 26 (Jul 1989) ISSN: 0376-2491 [Print] China
• PMID: 2557137 (Publication Type: English Abstract, Journal Article)

Chemical References

• Lectins
• Membrane Glycoproteins

Topics

• Adenocarcinoma (metabolism)
• Adenocarcinoma, Mucinous (metabolism)
• Adult
• Female
• Humans
• Immunohistochemistry
• Lectins
• Male
• Membrane Glycoproteins (metabolism)
• Middle Aged
• Stomach Neoplasms (metabolism)