[Ephrin B2 is involved in Porphyromonas gingivalis infection-enhanced adhesion of THP-1 to human umbilical vein endothelial cells].

Abstract

OBJECTIVE:

To investigate the mechanisms of Porphyromonas gingivalis (Pg) infection-mediated enhancement of adhesion between monocytes THP-1 and human umbilical vein endothelial cells (HUVEC) by detecting the effect of erythropoietin producing hepatomocellular receptor interacting protein B2 (Ephrin B2) and its receptors on the adhesion.

METHODS:

PgATCC33277 was cultured in an anaerobic jar, and THP-1 cells were infected with various concentrations of Pg at multiplicity of infection (MOI) of 1:100 for 8 and 24 h, respectively. The expression of Ephrin B2 receptor of THP-1 cells was detected. After removal of the free Pg, THP-1 cells were cocultured with HUVEC (overexpress of EphrinB2 or not) for 24 h to detect the expression of Ephrin B2 of HUVEC cells after additional cultivation for 23 h.

RESULTS:

The adhesion of THP-1 cells post infection by Pg to HUVEC was enhanced. The mRNA levels of Ephrin B2 receptors, including EphB3 (5.169±0.152, P = 0.005), EphB4 (11.040±1.195, P = 0.001), and EphA4 (4.976± 0.122, P = 0.001) expressed by THP-1, and Ephrin B2 (8.938±0.962, P = 0.008) expressed by HUVEC were significantly elevated 24 h post infection of Pg. Over expression of Ephrin B2 in HUVEC promoted the adhesion of THP-1 to HUVEC.

CONCLUSIONS:

Ephrin B2 and its receptors are involved in Pg infection mediated enhancement of the adhesion of THP-1 to HUVEC cells, suggesting that Ephrin B2 participates in the development of atherosclerosis.

• Authors: Caixia Zhang, Jie Song, Yang Xu, Juan Wu, Weibin Sun, Kuanyu Li
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 49 Issue 10 Pg. 614-8 (Oct 2014) ISSN: 1002-0098 [Print] China
• PMID: 25567715 (Publication Type: Journal Article)

Chemical References

• Ephrin-B2
• Recombinant Proteins
• Erythropoietin
• Epoetin Alfa

Topics

• Atherosclerosis
• Cell Adhesion
• Cells, Cultured
• Coculture Techniques
• Endothelium, Vascular
• Ephrin-B2 (physiology)
• Epoetin Alfa
• Erythropoietin
• Human Umbilical Vein Endothelial Cells
• Humans
• Monocytes
• Porphyromonas gingivalis (pathogenicity)
• Recombinant Proteins
• Umbilical Veins (cytology)