Cholesterol and bile acid synthesis in Hep G2 cells. Metabolic effects of 26- and 7 alpha-hydroxycholesterol.

Abstract

1. Using a human hepatoma (Hep G2) cell line that continually synthesizes 3 beta-hydroxy-5-cholenoic acid, lithocholic acid, chenodeoxycholic acid and cholic acid we have determined the metabolism and biological effects of 26-hydroxycholesterol and 7 alpha-hydroxycholesterol. 2. Addition of 26-hydroxycholesterol to the medium (6 microM) downregulated cholesterol and chenodeoxycholic acid synthesis. 3. The predominant metabolite of 26-hydroxycholesterol was 3 beta-hydroxy-5-cholenoic acid. 4. Cholesterol synthesis was not affected by the addition of 7 alpha-hydroxycholesterol (6 and 12 microM). The predominant metabolite of 7 alpha-hydroxycholesterol was chenodeoxycholic acid. 5. In Hep G2 cells 7 alpha-hydroxylation of 26-hydroxycholesterol is not well expressed.

• Authors: N B Javitt, K Budai
• Journal: The Biochemical journal (Biochem J) Vol. 262 Issue 3 Pg. 989-92 (Sep 15 1989) ISSN: 0264-6021 [Print] England
• PMID: 2556116 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bile Acids and Salts
• Cholic Acids
• Hydroxycholesterols
• Chenodeoxycholic Acid
• cholest-5-ene-3 beta,26-diol
• 3 beta-hydroxy-delta 5-cholenic acid
• cholest-5-en-3 beta,7 alpha-diol
• Cholesterol

Topics

• Bile Acids and Salts (biosynthesis)
• Carcinoma, Hepatocellular (metabolism)
• Cell Line
• Chenodeoxycholic Acid (biosynthesis)
• Cholesterol (biosynthesis)
• Cholic Acids (metabolism)
• Humans
• Hydroxycholesterols (pharmacology)
• Liver Neoplasms (metabolism)