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Transcriptional mapping and nucleotide sequence of a vaccinia virus gene encoding a polypeptide with extensive homology to DNA ligases.

Abstract
Nucleotide sequencing of the vaccinia virus SalI F DNA fragment identified an open reading frame of 552 amino acids encoding a protein of 63.3 kDa. The deduced amino acid sequence shares 30% identity with S. pombe and S. cerevisiae DNA ligases, with homology strongest near the carboxy terminus and around the lysine residue required for ligase-adenylate formation. Prokaryotic DNA ligases are poorly related to the vaccinia sequence. The initiation codon of the ORF forms part of a late transcriptional initiation sequence TAAATG and is preceded by two overlapping early transcriptional termination signals, TTTTTTTAT. Nonetheless, RNA mapping showed that the ligase gene is transcribed early during infection and the 5' end of the mRNA maps to the TAAATG motif. The possible roles of a DNA ligase in vaccinia virus DNA replication and recombination are discussed.
AuthorsG L Smith, Y S Chan, S M Kerr
JournalNucleic acids research (Nucleic Acids Res) Vol. 17 Issue 22 Pg. 9051-62 (Nov 25 1989) ISSN: 0305-1048 [Print] England
PMID2555782 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Viral
  • Oligonucleotide Probes
  • Peptides
  • RNA, Viral
  • Viral Structural Proteins
  • Nucleotidases
  • DNA Ligases
  • Polynucleotide Ligases
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Cosmids
  • DNA Ligases (genetics)
  • DNA, Viral (genetics)
  • Genes, Viral
  • Molecular Sequence Data
  • Mutation
  • Nucleotidases
  • Nucleotide Mapping
  • Oligonucleotide Probes
  • Peptides (genetics)
  • Polynucleotide Ligases (genetics)
  • RNA, Viral (genetics)
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Software
  • Transcription, Genetic
  • Vaccinia virus (enzymology, genetics)
  • Viral Structural Proteins (genetics)

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