Silk fibroin/gelatin electrospun nanofibrous dressing functionalized with astragaloside IV induces healing and anti-scar effects on burn wound.

Abstract

Functional wound dressing has provided new challenges for researchers who focus on burn to improve skin graft quality, reduce scarring, and develop a pluristratified dermal or epidermal construct of a burn wound. This study aimed to investigate the effect of a silk fibroin/gelatin (SF/GT) electrospun nanofibrous dressing loaded with astragaloside IV (AS) on deep partial-thickness burn wound. AS-loaded SF/GT-blended nanofibrous dressing was prepared by electrospinning nanotechnology. The optimal ratio (25:75) of silk fibroin to gelatin was further optimized by evaluating ATR-FTIR characteristics, mechanical properties, porosity, swelling rate, degradation, and release profile of the AS-loaded SF/GT nanofibrous dressing. In contrast to the blank control, the AS-loaded SF/GT nanofibrous dressing promoted cell adhesion and proliferation with good biocompatibility in vitro (p<0.01). This dressing also accelerated wound healing and inhibited scar formation in vivo by stimulating wound closure (p<0.05), increasing angiogenesis, regulating newly formed types of collagen, and improving collagen organization. These results showed that SF/GT nanofibrous dressing is a promising topical drug delivery system. Furthermore, AS-functionalized SF/GT nanofibrous dressing is an excellent topical therapeutic that could be applied to promote healing and elicit anti-scar effects on partial-thickness burn wound.

• Authors: Ying-Hui Shan, Li-Hua Peng, Xin Liu, Xi Chen, Jie Xiong, Jian-Qing Gao
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 479 Issue 2 Pg. 291-301 (Feb 20 2015) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 25556053 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Saponins
• Silk
• Triterpenes
• astragaloside A
• Gelatin
• Collagen
• Fibroins

Topics

• Animals
• Bandages
• Burns (complications, drug therapy, pathology)
• Cell Adhesion
• Cicatrix (etiology, prevention & control)
• Collagen (metabolism)
• Drug Delivery Systems
• Fibroins (chemistry)
• Gelatin (chemistry)
• Nanofibers
• Neovascularization, Physiologic (drug effects)
• Rats
• Rats, Sprague-Dawley
• Saponins (administration & dosage, pharmacology)
• Silk (chemistry)
• Skin (drug effects, pathology)
• Triterpenes (administration & dosage, pharmacology)
• Wound Healing (drug effects)