Abstract | BACKGROUND: METHODS: The study included 86 patients with CD, 25 patients with UC and 45 controls, blood donors. In CD group, 24/86 (28%) had B1 phenotype, 20/86 (23%) B2, 13/86 (15%) B3 and 29/86 (34%) B2 + B3. L1 involvement was present in 13/86 (15%), L2 in 13/86 (15%), L3 in 60/86 (70%). Serum anti-OmpC IgA, anti-GP2 IgG and IgA antibodies were investigated by means of ELISA. The data obtained were tested statistically by means of descriptive statistics, non-paired t-test, Mann-Whitney rank sum test, Spearman rank order correlation and Pearson product moment correlation using SigmaStat software. RESULTS: Anti-OmpC IgA were noted to be significantly higher in CD (median 32.6, inter-quartile range (IQR) 18.9-60.7) compared to the controls (median 18.3, IQR 11.1-23.1), p < 0.001. Anti-GP2 IgG were significantly higher in CD (median 13.9, IQR 8.6-25.6) compared to the controls (median 8.0, IQR 4.7-10.8), p < 0.001. Anti-GP2 IgA were significantly higher in CD (median 20.1, IQR 9.1-40.4) compared to the controls (median 9.8, IQR 5.6-16.9), p < 0.001. Significant difference was found in anti-OmpC IgA between UC (median 26.2, IQR 20.2-36.4) and the controls (median 18.3, IQR 11.1-23.1), p < 0.001. In CD anti-OmpC IgA were significantly higher in B2 compared to B1: p = 0.041 and in B2 + B3 compared to B1: p = 0.036. Anti-GP2 IgA were significantly higher in B2 + B3 compared to B1: p = 0.009 and in B3 compared to B1: p = 0.029. In CD there was a significant difference in anti-OmpC IgA between patients with surgery and without surgery, p = 0.005. CONCLUSIONS: We have confirmed association between anti-OmpC IgA and IBD (CD and UC) and an association between anti-GP2 ( IgG and IgA) and CD. Patients with complicated forms of CD have significantly higher levels of anti-OmpC IgA and anti-GP2 IgA.
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Authors | Darina Kohoutova, Marcela Drahosova, Paula Moravkova, Stanislav Rejchrt, Jan Bures |
Journal | BMC gastroenterology
(BMC Gastroenterol)
Vol. 14
Pg. 190
(Dec 31 2014)
ISSN: 1471-230X [Electronic] England |
PMID | 25551469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycoproteins
- Immunoglobulin A
- Immunoglobulin G
- OmpC protein
- Porins
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Topics |
- Adult
- Aged
- Crohn Disease
(diagnosis, immunology)
- Female
- Glycoproteins
(immunology)
- Humans
- Immunoglobulin A
(blood)
- Immunoglobulin G
(blood)
- Inflammatory Bowel Diseases
(diagnosis, immunology)
- Male
- Middle Aged
- Porins
(immunology)
- Prospective Studies
- Young Adult
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