Comparative effects between fucoxanthinol and its precursor fucoxanthin on viability and apoptosis of breast cancer cell lines MCF-7 and MDA-MB-231.

Abstract

BACKGROUND/AIM:

We evaluated whether low doses of the natural carotenoid fucoxanthin and/or of its metabolite fucoxanthinol are effective against proliferation of estrogen-sensitive MCF-7 and estrogen-resistant MDA-MB-231 breast cancer cell lines.

MATERIALS AND METHODS:

These cell lines were stimulated with 10 to 20 μM fucoxanthin and/or fucoxanthinol, followed by cell viability assays, Annexin V immunofluorescence to evaluate apoptosis, as well as mRNA and protein extractions for changes in nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) members' expressions and nuclear translocations.

RESULTS:

Fucoxanthin and fucoxanthinol reduced the viability of MCF-7 and MDA-MB-231 cells in a time-dependent manner as a result of increased apoptosis. In both cell lines, modulatory actions of fucoxanthinol on members of the NF-κB pathway were more pronounced than that of fucoxanthin.

CONCLUSION:

In MDA-MB-231 cells, fucoxanthinol reduced nuclear levels of NF-κB members' p65, p52 and RelB. Fucoxanthinol and fucoxanthin could be effective for the treatment and/or prevention of breast cancer.

• Authors: Arlette Rwigemera, Jean Mamelona, Luc J Martin
• Journal: Anticancer research (Anticancer Res) Vol. 35 Issue 1 Pg. 207-19 (Jan 2015) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25550553 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Antineoplastic Agents
• Apoptosis Regulatory Proteins
• NF-kappa B
• SOX9 Transcription Factor
• SOX9 protein, human
• Xanthophylls
• beta Carotene
• fucoxanthin
• fucoxanthinol

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Breast Neoplasms
• Cell Nucleus (metabolism)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Drug Screening Assays, Antitumor
• Female
• Gene Expression (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• MCF-7 Cells
• NF-kappa B (genetics, metabolism)
• SOX9 Transcription Factor (genetics, metabolism)
• Xanthophylls (pharmacology)
• beta Carotene (analogs & derivatives, pharmacology)