Abstract | BACKGROUND: METHODS:
Burkitt's lymphoma cells were pharmacologically treated with niltubacin, tubacin or sodium butyrate (NaB) or transfected with siRNAs to knock down the expression of HDAC6. Cell migration and invasion ability were measured by transwell assay, and cell cycle progression was analyzed by flow cytometry. Cell adhesion and proliferation was determined by CellTiter-Glo luminescent cell viability assay kit. Cell morphological alteration and microtubule stability were analyzed by immunofluorescence staining. Effect of niltubacin, tubacin and NaB on acetylated tubulin and siRNA efficacy were measured by western blotting. RESULTS: Suppression of histone deacetylase 6 activity significantly compromised the migration and invasion of Burkitt's lymphoma cells, without affecting cell proliferation and cell cycle progression. Mechanistic study revealed that HDAC6 modulated chemokine induced cell shape elongation and cell adhesion probably through its action on microtubule dynamics. CONCLUSIONS:
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Authors | Ning Ding, Lingyan Ping, Lixia Feng, Xiaohui Zheng, Yuqin Song, Jun Zhu |
Journal | Cancer cell international
(Cancer Cell Int)
Vol. 14
Issue 1
Pg. 139
( 2014)
ISSN: 1475-2867 [Print] England |
PMID | 25546298
(Publication Type: Journal Article)
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