The disposition kinetics study of
cefquinome was conducted following single intravenous (IV) administration of 2mg/kg bodyweight in buffalo calves after oral subchronic exposure to
flubendiamide and to determine the in vitro
plasma protein binding of
cefquinome. Plasma concentrations of
cefquinome were analyzed using reverse-phase high performance liquid chromatography (HPLC). The results were compared with our earlier study on the pharmacokinetics of
cefquinome in untreated buffalo calves. Plasma concentration-time data for
cefquinome following IV injection were best fit into a two-compartmental open model in
flubendiamide-exposed buffalo calves. Following
flubendiamide exposure, most of the pharmacokinetic parameters of
cefquinome were significantly altered in buffalo calves.
Cefquinome was bound to
plasma proteins of buffalo calves to the extent of 11.4±0.66%. In
flubendiamide-exposed animals an intravenous dose of 2mg/kg
body weight would maintain the therapeutic plasma levels required to be effective against the bacterial pathogens with MIC values ≤0.39μg/mL for only 12h, whereas in untreated buffalo calves the same dose of 2mg/kg
body weight would maintain the plasma levels up to 24h, The study revealed that subchronic
flubendiamide exposure significantly alters the disposition of
cefquinome in buffalo calves.